Skip to main content
. 2016 Mar 1;24(2):123–131. doi: 10.4062/biomolther.2015.106

Fig. 6.

Fig. 6.

(A) Effects of aloin on MAPK signaling pathway. MC3T3-E1 cells were treated with or without aloinand analyzed for (a) p38, (b) phospho-p38, (c) JNK/SAPK, (d) phospho- JNK/SAPK, (e) ERK1/2 and (f) phospho-ERK1/2 by western blotting. β-actin was used as housekeeping protein. The data represent mean ± SD of triplicate determinations. *p<0.05 vs. control cells. (B) Effect of inhibitors of p38 (SB203580) and SAPK/JNK (SP600125) on the activity of aloin: Cells were treated with aloin (0.05 μM) in thepresence of SB203580 or SP600125. Protein expressions of Bmp-2 and RunX 2 were assessed by WB analysis. β-actin was used as housekeeping protein. Data represents the results of three independent experiments and significant level (*) was calculated at p<0.05 vs. control cells.