Fig. 3.

NAR increases transcriptional activity of ATF3 gene. (A) HCT116 and SW480 cells were treated with NAR at the indicated concentrations for 24 h. Total RNA was isolated and RT-PCR was performed (B) The pATF3-1420/+34 construct (1 μg) was co-transfected with pRL-null vector (0.1 μg). The cells were treated with NAR at the indicated concentrations for 24 h and then luciferase activity was measured. ^*p<0.05 compared to cells without NAR. (C) HCT116 and SW480 cells were transfected with indicated ATF3 deletion promoter constructs (1 μg) with pRL-null vector (0.1 μg). The cells were treated with DMSO or 200 μM of NAR for 24 h and luciferase activity was measured. ^*p<0.05 compared to cells transfected with pATF3−1420/−318 (Fold induction was above 3.0 in HCT116 and 1.5 in SW480 cells).