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. 2016 Mar 1;24(2):163–170. doi: 10.4062/biomolther.2015.217

Fig. 4.

Fig. 4.

Effect of apigenin on IL-1β-induced secretion of MMP-3 and caseinolytic activity of MMP-3 in rabbit articular chondrocytes. Primary cultured rabbit articular chondrocytes were pretreated with varying concentrations (1, 10, 50, and 100 μM) of apigenin for 2 h and then stimulated with IL-1β (10 ng/mL) for 24 h. Culture supernatants were collected for measurement of both the levels of produced and secreted MMP-3 by western blot analysis and the proteolytic activity of MMP-3 by casein zymography. The fourth panel was black-and-white version of casein zymography. Three independent experiments were performed and the representative data were shown. The signal intensity of each band was analyzed by GelQuant software (DNR Bio-Imaging Systems Ltd., Jerusalem, Israel). Each bar represents a mean ± S.E.M. of three independent experiments in comparison with that of the control set at 100%. *Significantly different from control (p<0.05). †Significantly different from IL-1β alone (p<0.05). Cont: control, concentration unit is μM.