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. Author manuscript; available in PMC: 2016 May 12.
Published in final edited form as: Biochemistry. 2015 May 1;54(18):2903–2910. doi: 10.1021/acs.biochem.5b00210

Figure 1.

Figure 1

Time course of BsQueE reaction under various reducing conditions. Activity was monitored by measuring CDG formation. BsQueE activity was measured in the presence 10 mM dithionite (upside-down triangle) or 2 μM Fpr/2 mM NADPH with 5 μM BsYkuN (circle), 5 μM Fpr/2 mM NADPH with 20 μM BsYkuP (triangle), or 5 μM Fpr/2 mM NADPH with 20 μM EcFldA (diamond). Assays where Fpr or NADPH (square) were omitted served as negative controls.