Table 1.

BsQueE kinetic parameters determined using various reduction conditions.

	EcFldA			BsYkuN			BsYkuP
	Dithionite	5 μM Fpr	20 μM Fpr	Dithionite	1 μM Fpr	5 μM Fpr	Dithionite	5 μM Fpr	20 μM Fpr
KFld (μM)	3.9 ± 1.4	29.0 ± 3.0	42.0 ± 9.0	0.13 ± 0.05a	0.3 ± 0.1a	0.09 ± 0.03a	2.0 ± 0.2	2.1 ± 0.2	1.1 ± 0.1
kCDG (min−1)	1.8 ± 0.5	4.2 ± 0.7	4.9 ± 0.8	14.6 ± 1.0	12.4 ± 1.1	12.4 ± 1.1	16.5 ± 0.7	10.7 ± 3.4	9.7 ± 2.7
kdAdo (min−1)	0.28	n.d.c	n.d.c	0.26	n.d.c	n.d.c	0.26	n.d.c	n.d.c
$\frac{{\mathit{k}}_{\mathit{CDG}}}{{\mathit{K}}_{\mathit{Fld}}}$ (μM−1min−1)	0.46 ± 0.21	0.14 ± 0.03	0.12 ± 0.03	110 ± 40b	41 ± 14b	140 ± 50b	8.3 ± 1.9	5.1 ± 1.7	8.8 ± 2.6
$\frac{{\mathit{k}}_{\mathit{CDG}}}{{\mathit{k}}_{\mathit{dAdo}}}$	6.4	n.d.c	n.d.c	56	n.d.c	n.d.c	64	n.d.c	n.d.c

^aThese values should be considered a lower limit for because at the lowest concentration of BsYkuN significant activity was observed.

^bGiven the uncertainties in the K_Fld value, these should be considered lower limits.

^cn.d. not detectable.