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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Dev Dyn. 2016 Jan 8;245(3):414–426. doi: 10.1002/dvdy.24376

Fig. 2.

Fig. 2

Gpr177 is required for facial development. A,B: Foxg1-Cre mediated loss of ectodermal Gpr177 leads to the disruption of craniofacial structures as revealed by gross morphology. C,D: Alizarin Red and Alcian Blue staining of skeletons on embryos at E16.5. E–L: Scanning electron micrographs of the facial prominences at E9.5 and E10.5. Front facial views of wild-type (E,G,J) and Gpr177-deficient (F,H,K). Gpr177Foxg1-Cre embryos display dramatic defects in nasal pit invagination (arrowhead in K vs. J) and outgrowth of mnp, lnp, and mxp (K vs. J) at E10.5. In contrast, mesenchymal deletion of Gpr177 by Wnt1-Cre shows minor defects with formation of the nasal pit (arrowhead in L). lnp, lateral nasal process; mnp, medial nasal process; mx, maxillary process; md, mandibular process. Scale bars ¼ 500 mm in E–I; 200 mm in J–L.