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. 2016 Feb 9;11(3):2009–2018. doi: 10.3892/ol.2016.4220

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Copyright: © Matuszewska et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Cytotoxic effect of ex-LAC on Jurkat cells. (A) Control cells. (B) Cells treated with 666.667 µg/ml ex-LAC. (C) Cells treated with 66.667 µg/ml ex-LAC. Cytotoxicity was assessed by staining the cells with Annexin V and PI prior to being subjected to flow cytometry analysis. In each graph, the lower left quadrant (R4) indicates viable cells (Annexin V⁻PI⁻); the upper left quadrant (R2) represents necrotic cells (Annexin V⁻PI⁺); the lower right quadrant (R5) indicates early apoptotic cells (Annexin V⁺PI⁻); and the upper right quadrant (R3) represents late apoptotic cells (Annexin V⁺PI⁺). ex-Lac, extracellular laccase; PI, propidium iodide.