Table II.
Percentage of live, apoptotic and necrotic Jurkat cells following 48-h treatment with Cerrena unicolor ex-LAC, as determined by flow cytometry using Annexin V and propidium iodide staining.
| Concentration of ex-LAC, µg/ml | Live cells, % | Apoptotic cells, % | Necrotic cells, % |
|---|---|---|---|
| 666.667 | 2.20 | 95.67 | 2.13 |
| 66.667 | 3.25 | 95.87 | 0.88 |
| 6.667 | 0.82 | 98.00 | 1.18 |
| 5.000 | 1.24 | 97.05 | 1.71 |
| 3.333 | 1.65 | 96.76 | 1.59 |
| 0.667 | 0.68 | 98.90 | 0.42 |
| 0.067 | 7.95 | 90.24 | 1.81 |
| 0.007 | 8.86 | 88.98 | 2.16 |
| Control | 99.93 | 0.05 | 0.02 |
ex-LAC, extracellular laccase.