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(A) Immunofluorescence in the four groups to detect CRT and HSP70 expression on the cell surface. (B) Immunoblotting of CRT, HSP70, and α-tubulin on the cell membrane fraction. (C) ELISA detection of HMGB1 secretion in supernatant. CRT, calreticulin; ADM, doxorubicin; HSP70, heat shock protein 70; DC, dendritic cell. *P<0.05.
