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. 2016 Mar 2;3:15057. doi: 10.1038/mtm.2015.57

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

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HexM is capable of hydrolyzing NBD-GM2 in a GM2AP-dependent manner. In cellulo hydrolysis of NBD-GM2 was accessed by HPTLC separation of the Folch-extracted NBD-glycolipids from HexM expressing (+HexM) and untransfected (-HexM) HEKHexABKO cells. The upper phase (a) contains acidic glycolipids (gangliosides) and the lower phase (b) contains primarily neutral glycolipids. Conduritol B epoxide (50 µmol/l), a nonreversible inhibitor of glucocerebrosidase, was also present in the medium to inhibit NBD-GlcCer degradation.²⁶ (c) In vitro assay of NBD-GM2 hydrolysis by equal MUGS units of three human Hex isozymes; HexBMM,²² HexA and HexM; in the absence (−) or presence (+) of human rGM2AP. (d) Quantitative comparison of hydrolysis of NBD-GM2 to NBD-GM3 by HexA and HexM (n = 2) expressed in arbitrary units (AU) and as a ratio of band intensities corresponding to NBD-GM3 and NBD-GM2 in HPTLC shown in panel c.