Table 1. Comparison of Km and Vmax values of HexM toward a neutral and a negatively charged artificial substrate with those of other Hex isozymes.
Hex | MUG Km (mmol/l) | MUG Vmaxa | MUGS Km (mmol/l) | MUGS Vmaxa | Reference |
---|---|---|---|---|---|
M (μμ)b | 1.10 ± 0.06 | 4.7 ± 0.1 | 0.21 ± 0.01 | 3.13 ± 0.03 | PRc |
A (αβ) | 0.67 ± 0.06 | 9.7 ± 0.3 | 0.26 ± 0.02 | 1.32 ± 0.03 | PR |
S (αα) | 1.5 ± 0.2 | 2.0 ± 0.1d | 0.3 ± 0.1 | 0.94 ± 0.04d | 7 |
B (ββ) | 0.71 ± 0.1 | 14.5 ± 0.5 | NDe | NDe | 7 |
Moles (MU) h−1 g−1 (Hex).
Indicates a homodimer of our α-based hybrid subunit.
Present report.
These values may be low due to the presents of significant amounts of inactive HexS (an unstable isozyme).
Not determined because of insufficient activity toward MUGS (MUG/MUGS ~1/300).