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. 2016 Mar 2;3:15057. doi: 10.1038/mtm.2015.57

Table 1. Comparison of Km and Vmax values of HexM toward a neutral and a negatively charged artificial substrate with those of other Hex isozymes.

Hex MUG Km (mmol/l) MUG Vmaxa MUGS Km (mmol/l) MUGS Vmaxa Reference
M (μμ)b 1.10 ± 0.06 4.7 ± 0.1 0.21 ± 0.01 3.13 ± 0.03 PRc
A (αβ) 0.67 ± 0.06 9.7 ± 0.3 0.26 ± 0.02 1.32 ± 0.03 PR
S (αα) 1.5 ± 0.2 2.0 ± 0.1d 0.3 ± 0.1 0.94 ± 0.04d 7
B (ββ) 0.71 ± 0.1 14.5 ± 0.5 NDe NDe 7
a

Moles (MU) h−1 g−1 (Hex).

b

Indicates a homodimer of our α-based hybrid subunit.

c

Present report.

d

These values may be low due to the presents of significant amounts of inactive HexS (an unstable isozyme).

e

Not determined because of insufficient activity toward MUGS (MUG/MUGS ~1/300).