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. 2016 Mar 2;3:16001. doi: 10.1038/mtm.2016.1

Figure 2.

Figure 2

Expression levels of coxsackievirus–adenovirus receptor (CAR), CD46, miR-142-3p, and human telomerase reverse transcriptase (hTERT) in the human cancer cell lines. (a) Surface expression levels of CAR and CD46 were assessed by flow cytometry. Open histograms represent isotype control staining. Filled histograms represent specific staining with anti-CAR antibody or anti-CD46 antibody. The data shown are representative of multiple measurements. (b) Expression levels of miR-142-3p were determined by real-time RT-PCR. The ratios of miR-142-3p to RNU6B levels were determined. The ratio of K562 cells was normalized to 1. (c) mRNA levels of hTERT were determined by real-time RT-PCR. The ratios of hTERT to GAPDH levels were determined. The ratio of H1299 cells was normalized to 1. Data are expressed as the mean ± SD (n = 3). ND, not detected.