Figure 3.

Green fluorescence protein (GFP) expression levels in the tumor cell lines following infection with the conditionally replicating Ads. (a) The cell suspensions were incubated with the conditionally replicating Ads at MOIs of 1 and 10. Following a 24-hour incubation, the GFP expression levels were determined by flow cytometry. Data are expressed as the mean ± SD (n = 3). *P < 0.001 in comparison with rAd-GFP. ^#P < 0.001 in comparison with rAdF35-GFP by one-way analysis of variance followed by Shapiro–Wilk’s analysis. Time-lapse images of (b) coxsackievirus–adenovirus receptor (CAR)-positive and (c) CAR-negative tumor cells following infection with the conditionally replicating Ads. (b) H1299 cells and (c) T24 cells were infected at an MOI of 10. Selected images taken at the indicated time points show cell morphology by phase-contrast microscopy (right panels) and GFP expression under fluorescence microscopy (left panels). Bar = 100 µm.