Skip to main content
. 2016 Mar 2;3:16001. doi: 10.1038/mtm.2016.1

Figure 7.

Figure 7

Detection of human cancer cells spiked into peripheral blood mononuclear cells (PBMCs) by the conditionally replicating Ads. H1299 and T24 cells stably expressing red fluorescent protein (RFP) were spiked into PBMCs. The cells were then infected with the Ads for 24 hours. Fluorescence images of (a) H1299 and (b) T24 cells following infection with the Ads. RFP-expressing H1299 and T24 cells (1 × 105 cells) were spiked into 5 × 106 PBMCs. The cells suspended in 1 ml of RPMI1640 medium were incubated with 2 × 107 IFU of the Ads for 24 hours. The expression of green fluorescence protein (GFP) and RFP was observed under a fluorescence microscope. White arrowheads indicate uninfected tumor cells. The images shown are representative of multiple experiments. Bar = 50 µm. (c) Efficiencies of GFP labeling of human cancer cells spiked into PBMCs. RFP-expressing H1299 and T24 cells were spiked into 2 × 106 PBMCs. The cells suspended in 1 ml of RPMI1640 medium were infected with 2 × 107 IFU of the Ads for 24 hours. Measurement of GFP and RFP expression in the cells was carried out by flow cytometry. Data are shown as the mean ± SD (n = 3).