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TRAF6 expression, K63-linked ubiquitination and constitutive activation of NF-κB. (A) Western blotting assay shows higher TRAF6 protein expression in the Calu-3 and SPC-A1 cells than that in the A549 and H1650 cells. (B) Western blotting revealed K63-linked ubiquitination of TRAF6 in the SPC-A1 cells. (C and D) EMSA and western blotting revealed constitutive activation of NF-κB in the SPC-A1 cells. *P<0.05 vs. A459 cells; **P<0.01 vs. A549 cells.
