Figure 3.

siRNA-induced TRAF6 knockdown reduces NF-κB activity in the SPC-A1 cells. Decreased NF-κB activation was detected in the TRAF6 siRNA-transfected SPC-A1 cells but not in the Calu-3 cells. SPC-A1 and Calu-3 cells were transfected with TRAF6 siRNA or control siRNA. After 24 or 48 h, cell extracts were immunoblotted to detect the indicated protein. Cytoplasmic lysates were analyzed for TRAF6 expression by western blotting, and β-actin was used as a loading control. Nuclear lysates were analyzed for P65 by western blotting assay and Lamin B was used as a loading control. Lane 1, TRAF6 siRNA; lane 2, non-transfected; lane 3, control siRNA. ^*P<0.05 vs. the non-transfected group; ^**P<0.01 vs. the non-transfected group.