Figure 6.

siRNA-induced TRAF6 knockdown decreases CD24 and CXCR4 expression in the SPC-A1 cells. SPC-A1 cells were transfected by TRAF6 siRNA or control siRNA. After 48 h, cell extracts were immunoblotted to detect the indicated protein. β-actin was used as a loading control. Lane 1, TRAF6 siRNA; lane 2, non-transfected; lane 3, control siRNA. (A) Western blot analysis shows the protein expression of MMP-9, MMP-1, MMP-2, Twist, TIMP-2, Slug, CD24, CXCR4 and β-tubulin. (B) Relative protein expression of CD24 and CXCR4 in non-transfected cells and cells transfected with control siRNA and TRAF6 siRNA. 1, TRAF6 siRNA; 2, non-transfected; 3, control siRNA. ^**P<0.01 vs. the non-transfected group.