Table I.

Primer sequences for PCR, MSP and bisulfite sequencing.

Genes		Primer pairs (5′-3′)	Product size (bp)
P16	F	GCCGATCCAGGTCATGATGAT
	R	GCATCTATGCGGGCATGGTTA	300
RASSF1A	F	TGGGGAGGTGAACTGGGAC
	R	ACACGGCACGCACTTGG	217
ppENK	F	GCGGTTCCTGACACTTTGC
	R	GGGTGCTGGTGCCATCTT	245
DNMT1	F	GACCCATCTCTTGAAGGTGGTGTT
	R	CCTCGTCATAACTCTCCACCTGCT	164
DNMT3a	F	AGGTGGACCGCTACATTGCC
	R	GAGATGTCCCTCTTGTCACTAACG	143
P16	MF	TTATTAGAGGGTGGGGCGGATCGC
	MR	GACCCCGAACCGCGACCGTAA	150
	UF	TTATTAGAGGGTGGGGTGGATTGT
	UR	CAACCCCAAACCACAACCATAA	151
RASSF1A	MF	GTGTTAACGCGTTGCGTATC
	MR	AACCCCGCGAACTAAAAACGA	93
	UF	TTTGGTTGGAGTGTGTTAATGTG
	UR	CAAACCCCACAAACTAAAAACAA	105
ppENK	MF	TGTGGGGAGTTATCGAGC
	MR	GCCTTCGCGAAAAAAATCG	96
	UF	TTGTGTGGGGAGTTATTGAGT
	UR	CACCTTCACAAAAAAAATCAATC	100
P16	BS-F	TTGTTGTTTAGGTTGGAGTGTAGTG
	BS-R	TCAAAAACATATATTAATAACAACCATCAA	256
ppENK	BS-F	AAAGAGTTTTTGGAAATAGGGGATA
	BS-R	CATCAACAATTTCCCACTAAAAAAT	241
RASSF1A	BS-F	GTATGTAAGGGTTGGATGTGTAGAGA
	BS-R	CCCCAAATAAAATCTCCACAAAAATC	298

MSP, methylation-specific PCR; F, forward; R, reverse; M, methylated; U unmethylated; BS, bisulfite sequence.