Table-2.
Virus titration and expression analysis of plaque derived viruses.
| hAd5/P1-2AB3BCwt | hAd5/P1-2AB3BCm | ||||
|---|---|---|---|---|---|
| Sample ID | Virus titer (log10TCID50/ml) | Mean O.D±SD (492 nm) | Sample ID | Virus titer (log10TCID50/ml) | Mean OD±SD (492 nm) |
| Plaque 1 | 6.69 | 0.124±0.001 | Plaque 1 | 7.50 | 0.239±0.001 |
| Plaque 2 | 6.23 | 0.425±0.003 | Plaque 2* | 8.33 | 0.273±0.004 |
| Plaque 3 | 5.00 | 0.161±0.006 | Plaque 3 | 8.00 | 0.291±0.005 |
| Plaque 4* | 7.50 | 0.248±0.002 | Plaque 4 | 5.67 | 0.496±0.006 |
| Plaque 5 | 6.50 | 0.183±0.003 | Plaque 5 | 7.50 | 0.144±0.004 |
| Plaque 6 | 5.67 | 0.361±0.006 | Plaque 6 | 6.69 | 0.378±0.002 |
| Plaque 7 | 6.23 | 0.215±0.007 | Plaque 7 | 8.00 | 0.261±0.002 |
| Plaque 8 | 7.38 | 0.305±0.003 | Plaque 8 | 7.56 | 0.201±0.006 |
| Plaque 9 | 7.32 | 0.325±0.003 | Plaque 9 | 6.23 | 0.210±0.001 |
| Plaque 10 | 6.00 | 0.219±0.002 | Plaque 10 | 8.00 | 0.239±0.006 |
*
Denotes that this virus sample was selected for preparation of seed virus stock