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Titration of the recombinant hAd5 viruses at various passage level.
| Sample ID | Virus titer (log10TCID50/ml) | |||||
|---|---|---|---|---|---|---|
| P1 | P2 | P3 | P4 | P5 | P6 | |
| hAd5/P1-2AB-3BCwt | ||||||
| Cell lysate | 8.67 | 9.60 | 10.00 | 9.50 | 9.8 | 9.67 |
| CsCl purified | NA | 10.70 | 10.77 | 10.67 | 10.7 | 10.60 |
| hAd5/P1-2AB3BCm | ||||||
| Cell lysate | 9.00 | 9.85 | 9.60 | 9.77 | 9.50 | 9.65 |
| CsCl purified | NA | 10.79 | 10.33 | 10.50 | 10.69 | 10.42 |
NA=Not applicable, means that the virus was not purified
