Table-2.
Mean (±SE) values (%) of segment wise sperm abnormalities in Surti buffalo bulls’ semen at different stages of cryopreservation (−196°C) in different extenders
| Freezing stage | Extender | Sperm segments | Overall | ||
|---|---|---|---|---|---|
| Head | Mid-piece | Tail | |||
| Initial | - | 1.83a±0.05 | 0.88a±0.03 | 3.44a±0.05 | 6.15a±0.08 |
| Prefreeze | TFYG | 2.27p±0.08 | 1.23q±0.06 | 4.40q±0.07 | 7.90q±0.14 |
| Bioxcell | 2.19p±0.09 | 1.56p±0.07 | 4.85p±0.12 | 8.60p±0.16 | |
| Optixcell | 1.79q±0.08 | 1.08q±0.08 | 4.21q±0.11 | 7.08r±0.15 | |
| Average | 2.08b±0.05 | 1.29b±0.04 | 4.49b±0.06 | 7.86b±0.10 | |
| Postthaw | TFYG | 3.42x±0.09 | 2.08x±0.07 | 6.83x±0.10 | 12.33x±0.17 |
| Bioxcell | 3.48x±0.10 | 2.23x±0.09 | 7.06x±0.12 | 12.75x±0.21 | |
| Optixcell | 3.02y±0.11 | 1.79y±0.07 | 6.46y±0.12 | 11.27y±0.18 | |
| Average | 3.31c±0.06 | 2.03c±0.05 | 6.78c±0.07 | 12.12c±0.12 | |
Means bearing different superscripts between freezing stages (a,b,c) and between extenders at prefreeze (p,q,r) and postthaw (x,y,z) stage differ significantly (p<0.05) TFYG=Triscitratefructoseegg yolkglycerol, SE=Standard error