Table-3.
Mean (±SE) values (%) of various types of acrosomal abnormalities in Surti buffalo bulls’ semen at different stages of cryopreservation (−196°C) in different extenders
| Freezing stage | Extender | Sperm acrosomal abnormalities | Overall | |||
|---|---|---|---|---|---|---|
| Swollen | Ruffled | Detached | Denuded | |||
| Initial | - | 2.29a±0.05 | 1.60a±0.06 | 1.10a±0.05 | 0.60a±0.05 | 5.60a±0.12 |
| Prefreeze | TFYG | 3.79q±0.11 | 2.90p±0.09 | 2.27±0.09 | 1.50p±0.09 | 10.46q±0.18 |
| Bioxcell | 4.21p±0.10 | 2.96p±0.08 | 2.52±0.08 | 1.69p±0.07 | 11.42p±0.22 | |
| Optixcell | 3.46r±0.12 | 2.56q±0.10 | 2.31±0.09 | 1.17q±0.05 | 9.48r±0.21 | |
| Average | 3.82b±0.07 | 2.81b±0.05 | 2.37b±0.05 | 1.45b±0.05 | 10.45b±0.14 | |
| Postthaw | TFYG | 8.50x±0.15 | 6.17y±0.11 | 5.13±0.13 | 3.31x±0.11 | 23.17y±0.23 |
| Bioxcell | 8.65x±0.15 | 6.71x±0.13 | 5.25±0.11 | 3.50x±0.11 | 24.10x±0.27 | |
| Optixcell | 7.81y±0.18 | 5.83z±0.11 | 5.00±0.12 | 2.81y±0.10 | 21.50z±0.25 | |
| Average | 8.32c±0.10 | 6.24c±0.07 | 5.13c±0.07 | 3.21c±0.07 | 22.92c±0.17 | |
Means bearing different superscripts between freezing stages (a,b,c) and between extenders at prefreeze (p,q,r) and postthaw (x,y,z) stage differ significantly (p<0.05), TFYG=Triscitratefructoseegg yolkglycerol, SE=Standard error