Figure-4.

Restriction digestion of recombinant plasmid with bam H1 and Sal I showing 309 bp insert (hfq) release from p Et32a (+) vector. Lane 1, 2, and 3: Insert release shown by arrow, Lane 4: 1 kb DNA ladder.

Restriction digestion of recombinant plasmid with bam H1 and Sal I showing 309 bp insert (hfq) release from p Et32a (+) vector. Lane 1, 2, and 3: Insert release shown by arrow, Lane 4: 1 kb DNA ladder.