Supplement Table-1.
Characteristics of microsatellites markers included in this study.
| Locusa | Chromosome number | Primer name | Primer sequences (5’→3’) Forward/Reverse/Forward FM13b |
Annealing temperature (C°) |
|---|---|---|---|---|
| INRA063 (D18S5) |
18 | INRA063F INRA063R INRA063FM13 |
ATTTGCACAAGCTAAATCTAACC AAACCACAGAAATGCTTGGAAG CACGACGTTGTAAAACGACATTTGCACAAGCTAAATCTAACC |
55 |
| INRA037 (D10S12) |
10 | INRA037F INRA037R INRA037FM13 |
GATCCTGCTTATATTTAACCAC AAAATTCCATGGAGAGAGAAAC CACGACGTTGTAAAACGACGATCCTGCTTATATTTAACCAC |
50 |
| MM12 (D9S20) |
9 | MM12F MM12R MM12FM13 |
CAAGACAGGTGTTTCAATCT ATCGACTCTGGGGATGATGT CACGACGTTGTAAAACGACCAAGACAGGTGTTTCAATCT |
55 |
| HEL9 (D8S4) |
8 | HEL9F HEL9R HEL9FM13 |
CCCATTCAGTCTTCAGAGGT CACATCCATGTTCTCACCAC CACGACGTTGTAAAACGACCCCATTCAGTCTTCAGAGGT |
60 |
| HEL1 (D15S10) |
15 | HEL1F HEL1R HEL1FM13 |
CAACAGCTATTTAACAAGGA AGGCTACAGTCCATGGGATT CACGACGTTGTAAAACGACCAACAGCTATTTAACAAGGA |
55 |
| ETH10 (D5S3) |
5 | ETH10F ETH101R ETH10FM13 |
GTTCAGGACTGGCCCTGCTAACA CCTCCAGCCCACTTTCTCTTCTC CACGACGTTGTAAAACGAC GTTCAGGACTGGCCCTGCTAACA |
60 |
| ETH152 (D5S1) |
5 | ETH152F ETH152R ETH152FM13 |
TACTCGTAGGGCAGGCTGCCTG GAGACCTCAGGGTTGGTGATCAG CACGACGTTGTAAAACGACTACTCGTAGGGCAGGCTGCCTG |
55 |
| BM1818 (D23S21) |
23 | BM1818F BM1818R BM1818FM13 |
AGCTGGGAATATAACCAAAGG AGTGCTTTCAAGGTCCATGC CACGACGTTGTAAAACGAC AGCTGGGAATATAACCAAAGG |
55 |
| BM2113 (D2S26) |
2 | BM2113F BM2113R BM2113FM13 |
GCTGCCTTCTACCAAATACCC CTTCCTGAGAGAAGCAACACC CACGACGTTGTAAAACGACAGCTGCCTTCTACCAAATACCC |
55 |
| ETH225 (D9S1) |
9 | ETH225F ETH225R ETH225FM13 |
GATCACCTTGCCACTATTTCCT ACATGACAGCCAGCTGCTACT CACGACGTTGTAAAACGACGATCACCTTGCCACTATTTCCT |
55 |
| TGLA53 (D16S3) |
16 | TGLA53F TGLA53R TGLA53FM |
GCTTTCAGAAATAGTTTGCATTCA ATCTTCACATGATATTACAGCAGA CACGACGTTGTAAAACGACGCTTTCAGAAATAGTTTGCATTCA |
55 |
| TGLA122 (D21S6) |
21 | TGLA122F TGLA122R TGLA122M13 |
CCCTCCTCCAGGTAAATCAGC AATCACATGGCAAATAAGTACATAC CACGACGTTGTAAAACGACCCCTCCTCCAGGTAAATCAGC |
55 |
a
The codes for each locus on the genetic map of bovine genome are put in parentheses. Source: FAO (2011),
b
Forward primer whose sequence is provided with a tail M13 (sequence of 19 base pairs) to its 5 ‘end