Table-1.
List of primers, accession number and PCR product size.
| Primers | Nucleotide sequence | Accession no. | PCR product size (bp) | Reference |
|---|---|---|---|---|
| (1) 16 S rRNA | 16S rRNA F GTA GGT GGC AAG CGT TAT CC 16S rRNA R CGC ACA TCA GCG TCA G |
KM507158.1 | 228 | [11] |
| (2) PVL | PVL-F ATCATTAGGTAAAATGTCTGGACATGATCCA PVL-RGCATCAASTGTATTGGATAGCAAAAGC |
HM584707.1 | 433 | [12] |
| (3) CLFA | ClfA-F GGC TTC AGT GCT TGT AGG ClfA-R TTT TCA GGG TCA ATA TAA GC |
KJ001294.1 | 980 | [13] |
| (4) Coa | Coa –F CGA GAC CAA GAT TCA ACA AG Coa -R AAA GAA AAC CAC TCA CAT CA |
KJ746934.1 | Polymorphism 970, 910, 740, 410 | [14] |
| (5) Hla | HLA-1CTGATTACTATCCAAGAAATTCGATTG HLA-2CTTTCCAGCCTACTTTTTTATCAGT |
M90536 | 209 | [15] |
| (6) Hlb | HLB-1 GTGCACTTACTGACAATAGTGC HLB-2 GTTGATGAGTAGCTACCTTCAGT |
S72497 | 309 | [15] |
| (7) Enterotoxins | ||||
| SA-U (20) | 5’-TGTATGTATGGAGGTGTAAC-3’ Universal forward primer |
- | - | [16] |
| SA-A (18) | 5’-ATTAACCGAAGGTTCTGT-3’ Reverse primer for sea |
GQ859135.1 | 270 | |
| SA-B (18) | 5’-ATAGTGACGAGTTAGGTA-3’ Reverse primer for seb |
KC428707.1 | 165 | |
| SA-C (20) | 5’-AAGTACATTTTGTAAGTTCC-3’ Reverse primer for sec |
GQ461752.1 | 69 | |
| ENT-C (25) | 5’-AATTGTGTTTCTTTTATTTTCATAA-3’ Reverse primer for sec |
KF386012.1 | 102 | |
| SA-D (20) | 5’-TTCGGGAAAATCACCCTTAA-3’ Reverse primer for sed |
CP007455.1 | 306 | |
| SA-E (16) | 5’-GCCAAAGCTGTCTGAG-3’ Reverse primer for see |
EU604545.1 | 213 |
Hlb=Betahemolysin, Hla=Alphahemolysin, Coa=Coagulase, CLFA=Clumping factor A, PVL=Panton valentine leukocidin, PCR=Polymerase chain reaction