Table-4.
Confirmation of S. aureus isolates from cattle and buffaloes and molecular detection of some virulence factors by PCR using 16S rRNA, PVL, CLA coagulase gene, Hla, Hlb, and primers targeting genes of enterotoxins.
| Type of animal | No. of animals and isolates | Type of mastitis | PCR using primers to detect | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 16S rRNA (%) | PVL (%) | CLA (%) | Coa Gene (%) | Hla (%) | Hlb (%) | Enterotoxins targeting genes | |||
| Cattle | 11 | Clinical | 11/11 (100) | 6/11 (54.54) | 6/11 (36.4) | 11/11 (100) | 4/11 (36.4) | 7/11 (63.6) | -ve |
| 8 | Subclinical | 8/8 (100) | 7/8 (87.5) | 3/8 (37.5) | 8/8 (100) | 2/8 (25) | 3/8 (37.5) | 4/8 (50%) Type D toxin | |
| Buffaloes | 5 | Clinical | 5/5 (100) | 5/5 (100) | 4/5 (80) | 5/5 (100) | 1/5 (20) | 4/5 (80) | -ve |
| 8 | Subclinical | 8/8 (100) | 3/8 (37.5) | 3/8 (37.5) | 8/8 (100) | 4/8 (50) | 0 (0.0) | -ve | |
| Total | 32 | 32/32 (100) | 21/32 (65.6) | 16/32 (50) | 32/32 (100) | 11/32 (34.4) | 14/32 (43.75) | 4/32 (12.5%) | |
Hlb=Betahemolysin, Hla=Alphahemolysin, Coa=Coagulase, CLFA=Clumping factor A, PVL: Panton valentine leukocidin, PCR: Polymerase chain reaction, S. aureus=Staphylococcus aureus