Table-1.
Standard primers used for identification of T. annulata and T. orientalis (including type chitose, buffeli, and ikeda) along with their primer sequences, amplification targets, cycling conditions, and product size.
| Parasite | Primer sequence pair | Amplification target | Cycling conditions | Product size |
|---|---|---|---|---|
| T. annulata | TaF1:5’-GTA ACC TTT AAA AAC GT-3’ TaR1:5’-GTT ACG AAC ATG GGT TT-3’ |
30 kDa major T.annulata merozoite surface antigen gene |
94°C, 1 min 55°C, 1 min 72°C, 1 min 30 cycles |
721 bp |
|
T. orientalis (Entire
T. orientalis group) |
Tor: F1:5’-CTT TGC CTA GGA TAC TTC CT-3’ Tor: R1:5’-ACG GCA AGT GGT GAG AAC T-3’ |
Gene encoding a polymorphic MPSP |
95°C,15 sec 57°C,30 sec 72°C, 1 min 30 cycles |
776 bp |
|
T. orientalis
(Type-Chitose) |
TorC: F1:5’-GCG GAT CCT CAT CGT CTC TGC AAC T-3’ | SSUrRNA gene | 831 bp | |
|
T.orientalis
(Type-Buffeli) |
TorB: F1:5’-GCG GAT CCG CTC TGC AAC CGC AGA G-3’ | 825 bp | ||
| T.orientalis (Type-Ikeda) | TorI: F1:5’-AAG GAT CCG TCT CTG CTA CCG CCG C-3’ | 826 bp |
T. orientalis=Theileria orientalis, T. annulata=Theileria annulata, MPSP=Merozoite/piroplasms surface protein