Table-2.

Cycling conditions for the PCR assay.

Step	Temperature	Time	Cycle
Initial denaturation	95°C	10 min
Denaturation	95°C	30 sec	35 cycles
Primer annealing	59°C	60 sec
Extension	72°C	60 sec
Final extension	72°C	10 min

PCR=Polymerase chain reaction