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. 2016 Feb 16;72(Pt 3):165–171. doi: 10.1107/S2053230X16000960

Table 1. Macromolecule-production information.

Source organism Maize (Z. mays L.)
DNA source Leaf cDNA
Forward primer 5′-GCGGATCCATATGGGAAGTAAAGACGGCAC-3′
Reverse primer 5′-GTGCTCGAGGTGTTTGTACATAGACTGCCAG-3′
Cloning vector pUC18-T vector
Expression vector pet-22-ZmSR
Expression host E. coli BL21(DE3)
Complete amino-acid sequence of the construct produced§ MGSKDGTGDISEAQGYAADIDSIREAQARIAPYVHRTPVMSSTSIDAMVGKKLFFKCECFQKAGAFKIRGASNSIFALDDEQVSKGVVTHSSGNHAAAVALAAKLRGIPAHIVIPRNAPASKVENVKCYGGHIIWSDASIESREYVSKRVQEETGAVLIHPINSKYTISGQGTVSLELLEQVPEIDTIIVPISGGGLISGVALAAKAINPSIRILAAEPKGADDSAQSKAAGKIITLPSTNTIADGLRAFLGDLTWPVVRDLVDDVIVVDDTAIVDAMKMCYEILKVAVEPSGAIGLAAALSDEFKQSSAWHESSKIGIIVSGGNVDLGTLWQSMYKHLEHHHHHH

The NdeI site is underlined.

The XhoI site is underlined.

§

The two extra amino-acid residues and the C-terminal His6 tag are underlined.