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. 2016 Feb 16;72(Pt 3):165–171. doi: 10.1107/S2053230X16000960

Table 2. Crystallization.

Method Hanging-drop vapour diffusion
Plate type 24-well
Temperature (K) 277
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 20 mM Tris–HCl, 100 mM NaCl, 10% glycerol pH 8.0
Composition of reservoir solution 0.2 M calcium acetate, 0.1 M sodium cacodylate pH 6.5, 18% polyethylene glycol 8000
Volume and ratio of drop 1 µl; 1:1
Volume of reservoir (µl) 200