Table 1. Macromolecule-production information.

Protein	ZmPPX	ZmPPX(30–508)	ZmPPX(30–508) E137A mutant
Source organism	Z. mobilis subsp. mobilis NCIMB 11163 (GenBank accession No. CP001722.1)	Z. mobilis subsp. mobilis NCIMB 11163 (GenBank accession No. CP001722.1)	Z. mobilis subsp. mobilis NCIMB 11163 (GenBank accession No. CP001722.1)
DNA source	Plasmid	Plasmid	Plasmid
Forward primer	CGGGATCCATGATATTGAATAACAGGCAGG†	CGGGATCCATCGGTATCATTGATATTGGTT†	TCTTGTCGGGCGAAGAAGCGGGCTATGCT‡
Reverse primer	CCGCTCGAGTTATAAAAATTCGACCACTTCG§	CCGCTCGAGTTATAAAAATTCGACCACTTCG§	GCTTCTTCGCCCGACAAGACTTCAACTTC‡
Cloning vector	Modified pET-32a¶	Modified pET-32a	Modified pET-32a
Expression vector	Modified pET-32a	Modified pET-32a	Modified pET-32a
Expression host	E. coli BL21 (DE3)	E. coli BL21 (DE3)	E. coli BL21 (DE3)
Complete amino-acid sequence of the construct produced††	GPGSMILNNRQGSNDVDKKCPMPKQKKWCKSGPIGIIDIGSNSIRLVVYDQLSRAPRILFNEKISAQLGRNIPVDGRIDEKAIELAISELTRFWKLAQIMELSSLRTVATAAVRDAKNGAFLLGEIAKIGLEVEVLSGEEEGYASGYGVLSAIPDADGIVGDLGGGSLELIRVSKGRVKDRVSLPLGVLRIADIRKKSRNALDNFISEAFKKIDWLADARDLPFYMVGGAWRSLAKLDMHVRHYPIPVLHNYIMSPDRPSKLIRVIQRNNPKKLKNKANISTSRVEQLSDAAALLAVVSRHLHSRALVTSAYGLREGLLYLSLDKATRKLDPLLWSANQRGETAGRFYQQGEALYDWMSTLFAQDPPAYHRLRHAACLLADSAWQANPDFRAEQILSIILHGRWVGLDAYGRALIGQALAVSYDGAMDKKITNNLLSEADTIRAVRWGKAIRLGMRLSGGVTTSLKKSTILYRNNKIILQFSGNYKLKGETVLRRLRSLASSFEASEVVEFL	GPGSIGIIDIGSNSIRLVVYDQLSRAPRILFNEKISAQLGRNIPVDGRIDEKAIELAISELTRFWKLAQIMELSSLRTVATAAVRDAKNGAFLLGEIAKIGLEVEVLSGEEEGYASGYGVLSAIPDADGIVGDLGGGSLELIRVSKGRVKDRVSLPLGVLRIADIRKKSRNALDNFISEAFKKIDWLADARDLPFYMVGGAWRSLAKLDMHVRHYPIPVLHNYIMSPDRPSKLIRVIQRNNPKKLKNKANISTSRVEQLSDAAALLAVVSRHLHSRALVTSAYGLREGLLYLSLDKATRKLDPLLWSANQRGETAGRFYQQGEALYDWMSTLFAQDPPAYHRLRHAACLLADSAWQANPDFRAEQILSIILHGRWVGLDAYGRALIGQALAVSYDGAMDKKITNNLLSEADTIRAVRWGKAIRLGMRLSGGVTTSLKKSTILYRNNKIILQFSGNYKLKGETVLRRLRSLASSFEASEVVEFL	GPGSIGIIDIGSNSIRLVVYDQLSRAPRILFNEKISAQLGRNIPVDGRIDEKAIELAISELTRFWKLAQIMELSSLRTVATAAVRDAKNGAFLLGEIAKIGLEVEVLSGEEEGYASGYGVLSAIPDADGIVGDLGGGSLELIRVSKGRVKDRVSLPLGVLRIADIRKKSRNALDNFISEAFKKIDWLADARDLPFYMVGGAWRSLAKLDMHVRHYPIPVLHNYIMSPDRPSKLIRVIQRNNPKKLKNKANISTSRVEQLSDAAALLAVVSRHLHSRALVTSAYGLREGLLYLSLDKATRKLDPLLWSANQRGETAGRFYQQGEALYDWMSTLFAQDPPAYHRLRHAACLLADSAWQANPDFRAEQILSIILHGRWVGLDAYGRALIGQALAVSYDGAMDKKITNNLLSEADTIRAVRWGKAIRLGMRLSGGVTTSLKKSTILYRNNKIILQFSGNYKLKGETVLRRLRSLASSFEASEVVEFL

^†The BamHI site is underlined.

^‡The mutant site is underlined.

^§The XhoI site is underlined.

^¶Contains only the N-terminal 6×His tag and a PreScission Protease cleavage site.

^††Non-native residues originating from the expression vector are underlined.