| Method |
Hanging-drop vapour diffusion |
Sitting-drop vapour diffusion |
Sitting-drop vapour diffusion |
| Plate type |
48-well plates |
48-well plates |
48-well plates |
| Temperature (K) |
293 |
293 |
293 |
| Protein concentration (mg ml−1) |
10 |
8 |
8 |
| Buffer composition of protein solution |
50 mM Tris pH 8.0, 500 mM NaCl, 5% glycerol |
50 mM Tris pH 8.0, 500 mM NaCl, 5% glycerol |
50 mM Tris pH 8.0, 500 mM NaCl, 5% glycerol |
| Composition of reservoir solution |
15.2%(w/v) polyethylene glycol 3350, 2%(w/v) polyethylene glycol 8000, 72 mM Tris pH 8.0, 144 mM magnesium chloride hexahydrate, 20 mM HEPES/sodium hydroxide pH 7.5, 1.6%(v/v) glycerol, 8%(v/v) DMSO |
0.2 M magnesium chloride hexahydrate, 0.1 M Tris pH 8.5, 25%(w/v) polyethylene glycol 3350 |
0.2 M magnesium chloride hexahydrate, 0.1 M Tris pH 6.5, 25%(w/v) polyethylene glycol 3350 |
| Volume and ratio of drop |
3 µl in total, 2:1 |
2 µl in total, 1:1 |
2 µl in total, 1:1 |
| Volume of reservoir (µl) |
200 |
100 |
100 |
