Table 3. Data collection and processing.
Values in parentheses are for the outer shell.
| Protein | ZmPPX | ZmPPX(30–508) | ZmPPX(30–508) E137A mutant |
|---|---|---|---|
| Diffraction source | BL-17U1, SSRF | BL-19U1, SSRF | BL-17A, KEK |
| Wavelength (Å) | 0.98 | 0.98 | 0.98 |
| Temperature (K) | 100 | 100 | 100 |
| Detector | ADSC Quantum 315r | Pilatus 6M | ADSC Quantum 270 |
| Crystal-to-detector distance (mm) | 400.0 | 300.0 | 295.0 |
| Rotation range per image (°) | 0.5 | 0.5 | 0.5 |
| Total rotation range (°) | 180 | 267 | 180 |
| Space group | P2 | C2 | C2 |
| a, b, c (Å) | 58.3, 104.1, 242.2 | 122.0, 47.1, 89.5 | 122.7, 47.9, 94.9 |
| α, β, γ (°) | 90.0, 92.3, 90.0 | 90.0, 124.5, 90.0 | 90.0, 126.5, 90.0 |
| Resolution range (Å) | 50.0–3.30 (3.42–3.30) | 50.0–1.80 (1.84–1.80) | 50.0–1.56 (1.59–1.56) |
| Total No. of reflections | 162804 | 156187 | 206483 |
| No. of unique reflections | 43109 (4335) | 38980 (2498) | 63367 (3094) |
| Completeness (%) | 98.5 (99.1) | 98.9 (96.3) | 99.6 (98.2) |
| Multiplicity | 3.8 (3.8) | 4.0 (3.1) | 3.3 (2.9) |
| 〈I/σ(I)〉 | 16.8 (2.5) | 11.6 (2.0) | 26.2 (3.1) |
| R meas | 0.086 (0.499) | 0.136 (0.620) | 0.051 (0.375) |
| Overall B factor from Wilson plot (Å2) | 44.4 | 20.3 | 10.5 |