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. 2016 Feb 16;72(Pt 3):207–213. doi: 10.1107/S2053230X16002016

Table 2. Macromolecule-production information.

Source organism H. sapiens
DNA source Chemically synthesized (GenScript, USA)
Cloning vector pUC57
Expression vector pET-28b
Expression host E. coli strain BL21 Rosetta-gami (DE3)
Complete amino-acid sequence of the construct MGSSHHHHHHSSGLVPRGSHMGKIALQLKATLENITNLRPVGEDFRWYLKMKCGNCGEISDKWQYIRLMDSVALKGGRGSASMVQKCLCARENSIEILSSIKPYNAEDNENFKTIVEFECRGLEPVDFQPQAGFAVESGTAFSDINLQEKDWTDYDEKAQESVGIYEVTHQFVKC

The 6×His fusion-tag sequence at the N-terminus of recombinant C1ORF123 is underlined.