| Method |
Sitting-drop vapor diffusion (initial crystallization) and hanging-drop vapor diffusion (crystal optimization) |
| Plate type |
96-well MRC plates (initial crystal screening) and 24-well plates (crystal optimization) |
| Temperature (K) |
293 |
| Protein concentration (mg ml−1) |
8.0 (initial screening) and 7.8 (optimization) |
| Buffer composition of protein solution |
25 mM Tris–HCl, 0.1 M sodium chloride tribasic pH 7.5, 20 mM β-mercapthoethanol |
| Composition of reservoir solution |
0.2 M magnesium chloride hexahydrate, 0.1 M sodium citrate tribasic buffer pH 6.5, 20% polyethylene glycol 3350 |
| Volume and ratio of drop |
1 µl, 1:1 ratio of protein:reservoir solution (initial crystal screening); 2 µl, 1:1 ratio of protein:reservoir solution (crystal optimization) |
| Volume of reservoir |
80 µl (initial crystallization) and 1.0 ml (crystal optimization) |
