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. 2016 Feb 19;72(Pt 3):214–219. doi: 10.1107/S2053230X16002272

Table 2. Crystallization.

Method Sitting-drop vapour diffusion
Plate type Low-profile 96-well (Greiner)
Temperature (K) 293
Protein concentration (mg ml−1) 60
Buffer composition of protein solution 20 mM Tris–HCl pH 8.0, 150 mM NaCl
Composition of reservoir solution 0.1 M CdCl2, 30%(v/v) PEG 400, 0.1 M sodium acetate pH 4.6
Volume and ratio of drop 200 nl protein solution with 200 nl reservoir solution