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. 2016 Feb 19;72(Pt 3):224–233. doi: 10.1107/S2053230X1600162X

Table 2. Crystallization.

  Unliganded StTP StTP–THM StTP–URI
Method Sitting-drop vapour diffusion Sitting-drop vapour diffusion Sitting-drop vapour diffusion
Plate type 24-well plate 24-well plate 24-well plate
Temperature (K) 292 292 292
Protein concentration (mg ml−1) 19 19 19
Buffer composition of protein solution 20 mM Tris–HCl, 20 mM NaCl 20 mM Tris–HCl, 20 mM NaCl 20 mM Tris–HCl, 20 mM NaCl
Composition of reservoir solution 0.2 M potassium thiocyanate, 0.1 M bis-tris propane, 20%(w/v) PEG 3350 pH 7.5 0.1 M Bicine, 1.6 M (NH4)2SO4 pH 9.0 0.1 M citric acid, 10%(w/v) PEG 6000 pH 5.0
Volume and ratio of drop 2 µl protein solution + 2 µl reservoir solution 2 µl protein solution + 2 µl reservoir solution 2 µl protein solution + 2 µl reservoir solution
Volume of reservoir (µl) 300 300 300