| Method |
Sitting-drop vapour diffusion |
Sitting-drop vapour diffusion |
Sitting-drop vapour diffusion |
| Plate type |
24-well plate |
24-well plate |
24-well plate |
| Temperature (K) |
292 |
292 |
292 |
| Protein concentration (mg ml−1) |
19 |
19 |
19 |
| Buffer composition of protein solution |
20 mM Tris–HCl, 20 mM NaCl |
20 mM Tris–HCl, 20 mM NaCl |
20 mM Tris–HCl, 20 mM NaCl |
| Composition of reservoir solution |
0.2 M potassium thiocyanate, 0.1 M bis-tris propane, 20%(w/v) PEG 3350 pH 7.5 |
0.1 M Bicine, 1.6 M (NH4)2SO4 pH 9.0 |
0.1 M citric acid, 10%(w/v) PEG 6000 pH 5.0 |
| Volume and ratio of drop |
2 µl protein solution + 2 µl reservoir solution |
2 µl protein solution + 2 µl reservoir solution |
2 µl protein solution + 2 µl reservoir solution |
| Volume of reservoir (µl) |
300 |
300 |
300 |