Fig 6. In vitro evaluation of anti-TB therapeutic efficacy using the M. bovis BCG L5-tdTomato strain.
Fluorescence of treated or untreated M. bovis BCG L5-tdTomato strain was measured with a multimode reader. The initial CFU for the treated or untreated was 106. The treated group was given 64 ng/μl rifampicin and isoniazid. At each time point, bacteria were loaded into a 96-well plates as quadruplicate samples for each group. The Y-axes represent mean fluorescence for the quadruplicates. Data represents one of two independent replicate experiments. Error bars are standard deviations. Repeated Measures Two-way ANOVA was performed to examine overall difference between the two groups (treated vs. untreated) through multiple time points. Comparison of treated with untreated group was matched at each time point. P<0.01.