Fig 7. In vivo evaluation of anti-TB therapeutic efficacy using the M. bovis BCG L5-tdTomato strain.

A. Images of infected mice treated or untreated with anti-TB therapy. Mice were infected intratracheally with M. bovis BCG with L5-tdTomato and treated or untreated with anti-TB therapy (10 μg/g rifampicin and isoniazid; intraperitoneal injection.). Images were acquired by trans-illumination at day 0, 2, and 6 post infection. B. Quantitative analysis of in vivo images. C. Images of lungs harvested from the mice sacrificed at day 0, 2, and 6 post infection after in vivo imaging. Images were acquired by epi-illumination. D. Quantitative analysis of images of harvested lungs. E. Colony forming units (CFU) in mouse lungs recovered from agar plating. Y-axis is in log scale. There were four infected mice and one uninfected mouse per group per time point. Data represents two independent replicate experiments. Two-way ANOVA was performed to examine overall difference between the two groups (treated vs. untreated) through multiple time points. Bonferroni post-hoc tests have been performed between treated and untreated groups at each time point. * P<0.05; ** P<0.01; and ***P<0.01