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. 2016 Mar 2;11(3):e0150607. doi: 10.1371/journal.pone.0150607

Fig 2. Confocal microscopic images of inducible nitric oxide synthase (iNOS) in peritoneal macrophages isolated from borax treated mice.

Fig 2

Mice were treated with different doses of borax, B1, B2 and B3, as described in Materials and Methods section of the text. The isolated macrophages were incubated with primary iNOS antibody followed by incubation with Alexa Fluor® 488. Images were captured in a LEICA SP5II confocal microscope at 40x (magnification). Arrows indicate increase in fluorescence of iNOS conjugated with Alexa Fluor® 488. DAPI: 4',6-Diamidino-2-phenylindole. In all groups, except the untreated and unstimulated control group, cells were stimulated with lipopolysaccharide (LPS) after isolation from mouse peritoneum.