Table 5. Comparison of T3SS gene expression in LB broth with 5% albumin to LB Broth.

Gene	Proteina	LB broth/5% albumin:LB brothb	Average of Burn Pts: Healthy volunteerc
exoT	ExoT, effector protein	2	5
exoY	ExoY, effector protein	2	2
exsB	ExsB, T3SS regulation	2	3
pcrD	PcrD, translocation apparatus operon	1	4
pcrG	PcrG, translocation apparatus operon	3	4
pcrH	PcrH, translocation apparatus operon	2	6
pcrV	PcrV, translocation apparatus operon	2	6
popB	PopB, translocation apparatus operon	2	4
popD	PopD, translocation apparatus operon	2	4
popN	PopN, translocation apparatus operon	2	3
pscB	PscB, needle complex operon	2	15
pscC	PscC, needle complex operon	2	17
pscD	PscD, needle complex operon	2	10
pscE	PscE, needle complex operon	2	5
pscF	PscF, needle complex operon	2	5
pscG	PscG, needle complex operon	1	6
pscH	PscH, needle complex operon	1	14
pscJ	PscJ, needle complex operon	2	7
pscN	PscN, second needle complex operon	2	3

^aProduct names for PA14 genes were obtained from the MGH-ParaBioSys:NHLBI Program for Genomic Applications, Massachusetts General Hospital and Harvard Medical School, Boston, MA (http://pga.mgh.harvard.edu; accessed 10Nov2015) [45] made available by the Pseudomonas Genome Database (http://www.pseudomonas.com/; accessed 10Nov2015) [44].

^bExpression of genes within PA14 that was grown in in LB broth with 5% albumin was compared with expression when PA14 was grown in LB broth.

^cAverage of gene expression within PA14 grown in whole blood from the three severely burned patients was compared with expression when PA14 was grown in whole blood from a healthy volunteer calculated from results used to derive Fig 4.