Table 6. Differentially expressed genes of the phosphate regulon (Pho).
| Gene | Product name | Pt 1 | Pt 2 | Pt 3 |
|---|---|---|---|---|
| Functional classification(s) // gene ontology terms | ||||
| Pathways // Functional prediction(s)a | ||||
| phoU | Phosphate uptake regulatory protein PhoU | -19b | -21 | -24 |
| Transcriptional regulators; transport of small molecules; membrane proteins // Negative-regulation of positive chemotaxis | ||||
| // Phosphate transport system regulatory protein PhoU | ||||
| pstBc | Phosphate transporter ATP-binding protein | -17 | -23 | -21 |
| Transport of small molecules // Phosphate ion transmembrane; membrane; inorganic phosphate transmembrane transport activity; phosphate ion trans-membrane ATPase activity; ATP binding | ||||
| ABC transporters // Phosphate transport system permease protein 1; ABC transporter, phosphate import, PstB; AAA+ ATPase domain | ||||
| pstA | Phosphate ABC transporter permease | -34 | -34 | -36 |
| Transport of small molecules // Phosphate ion transmembrane transport; integral component of membrane; inorganic phosphate transmembrane transport activity | ||||
| ABC transporters // Phosphate transport system permease protein PstA | ||||
| pstC | Membrane protein component of ABC phosphate transporter | -17 | -15 | -18 |
| Transport of small molecules // Transport; membrane; protein binding | ||||
| ABC transporters // ABC transporter integral membrane type-1 domain profile; binding-protein-dependent transport system inner membrane component | ||||
| oprO | Pyrophosphate-specific outer membrane porin OprO precursor | -45 | -48 | -57 |
| Transport of small molecules; no GO terms listed | ||||
| // Phosphate-selective porin O and P | ||||
| oprP | Pyrophosphate-specific outer membrane porin OprP precursor | -14 | -5 | -4 |
| Transport of small molecules // no GO terms listed | ||||
| // Phosphate-selective porin O and P | ||||
| phoB | Two-component response regulator PhoB | -9 | -10 | -13 |
| Transcriptional regulators; two-component regulatory systems // Phosphate ion transport; bacterial-type flagellum-dependent swarming motility; positive-regulation of cell response to phosphate starvation; phosphorelay signal transduction system; regulation of transcription, DNA-templated; phosphorelay response regulator activity, DNA binding | ||||
| Two-component system // Signal transduction response regulator, phosphate regulon transcriptional regulatory protein PhoB; signal transduction response regulator, receiver domain; winged helix-turn-helix DNA-binding domain | ||||
| phoR | Two-component sensor PhoR | -5 | -5 | -6 |
| Two-component regulatory systems // Regulation of transcription, DNA-templated; phosphorylation; phosphorelay signal transduction system; integral component of membrane; protein histidine kinase activity; phosphorelay sensor kinase activity; transferase activity (phosphorus-containing groups); signal transducer activity; | ||||
| Two-component systems // Signal transduction histidine kinase EnvZ-like, dimerisation/phosphoacceptor domain; Signal transduction histidine kinase, phosphate regulon sensor PhoR | ||||
| phoQc | Two-component sensor PhoQ | -3 | -5 | -5 |
| Two-component regulatory systems // Signal transduction; phosphorylation; integral component of membrane; signal transducer activity; transferase activity (phosphorus-containing groups); phosphorelay sensor kinase activity | ||||
| Two-component systems // Signal transduction histidine kinase EnvZ-like, dimerisation/phosphoacceptor domain; Signal transduction histidine kinase, homodimeric domain | ||||
| phoP | Two-component response regulator PhoP | -5 | -7 | -7 |
| Transcriptional regulators; two-component regulatory systems // Regulation of transcription, DNA-templated; positive regulation of phospholipid biosynthetic process; phosphorelay signal transduction system; DNA binding | ||||
| Two-component system // Signal transduction response regulator, receiver domain; winged helix-turn-helix DNA-binding domain | ||||
| oprH | PhoP/Q and low Mg2+ inducible outer membrane protein | -12 | -13 | -15 |
| Adaptation, protection; transport of small molecules; membrane proteins // integral component of membrane, cell outer membrane; lipopolysaccharide binding | ||||
| // Outer membrane protein beta-barrel domain | ||||
| PA14_55770 | Phosphate transporter | 2 | 2 | 2 |
| Transport of small molecules; membrane proteins // Phosphate ion transport; membrane; inorganic phosphate transmembrane transport activity | ||||
| // Phosphate transporter family |
aProduct names, functional classification(s), gene ontology terms, pathways, and functional predictions for PA14 genes were obtained from the MGH-ParaBioSys:NHLBI Program for Genomic Applications, Massachusetts General Hospital and Harvard Medical School, Boston, MA (http://pga.mgh.harvard.edu; accessed 10Nov2015) [45] made available by the Pseudomonas Genome Database (http://www.pseudomonas.com/; accessed 10Nov2015) [44].
bGene expression within PA14 grown in whole blood from the three severely burned patients (Pt) was compared with expression when PA14 was grown in whole blood from a healthy volunteer.
cGenes found in operons: pstB/pstA/pstC (and related protein phoU); phoQ/phoP/oprH.