Fig 1. FAP selectively cleaves type I collagen.

(A) DQ type I collagen was incubated with FAP (1 μg/ml) for increasing times and the extent of substrate cleavage quantified by measurement of increasing fluorescence. (B) DQ type I collagen was incubated with active or PMSF inhibited FAP (1 μg/ml) and DQ type IV collagen was incubated with active FAP at the same concentration for 24 hr. Degradation of DQ collagens was quantified by measurement increasing fluorescence. Shown are the means ± SD of at least 3 experiments.