Fig 2. FAP-mediated cleavage of type I collagen enhances macrophage adhesion.

(A) MPMs were adhered to type I collagen that was pretreated with buffer, FAP, and FAP inhibited with PMSF. Non-adhered cells were removed by washing, and the number of attached cells quantified and expressed as a percentage of total cells plated. Shown are the means ± SD of 4 experiments. Results were compared by one-way ANOVA with Tukey’s post-hoc test. (B) MPMs were adhered to type I collagen pretreated with buffer or FAP. Non-adhered cells were removed by washing, and attached cells were fixed and stained with fluorescent phalloidin and DAPI. Representative images were digitally captured and the surface area of cells quantified. Scale bars = 30 μm. Shown are the means ± SD of cell surface area and percentage of cells displaying a surface area > 100 μm² from 3 experiments. Results were log-transformed and compared by one-way ANOVA with Dunnett’s post-hoc test. * indicates significant (p<0.05) difference from cells plated on buffer treated collagen.