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. 2016 Mar 2;11(3):e0149424. doi: 10.1371/journal.pone.0149424

Fig 1. Schematic overview presenting all expression vectors used in this study.

Fig 1

This overview describes the genetic elements of all expression vectors used in this study. The ORF603 and ORF1629 are essential recombination elements required for the flashBAC system [9,50]. Tn7 R/L and the gentamicin resistance are required for the Bac-to-Bac system (Life Technologies). Terminator sequences are shown in blue boxes, eGFP as model protein in green. The long arrow-like symbols indicate promoter regions. hr5 is a homologous region and functions as an enhancer. The blue short arrow-like symbols represent Flipase Recognition Target (FRT) sites needed for the Recombinase Mediated Cassette Exchange (RMCE) system. The vector backbones are derived from plasmid pIEx/Bac-5 (Novagen) except for the pFlpBtM-I vectors, which are described in Meyer et al. [29]. The single plasmid “polH (pFastBac)” is originating from pFastBac1 (Life Technologies).