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. 2016 Mar 2;11(3):e0150364. doi: 10.1371/journal.pone.0150364

Fig 2. In situ hybridization of genes encoding DUB enzymes on retinal cryosections.

Fig 2

Sections from wild-type C57BL/6J mouse retinas were hybridized using digoxigenin-labelled antisense riboprobes. Their corresponding sense riboprobes (negative controls) stained for the same length of time (lower panels in each row) are in the S1 Fig. The antisense Rhodopsin probe, which strongly labels the inner photoreceptor segment, was used as a positive control for the assay. RPE- Retinal pigmented epithelium; Phr- Photoreceptor cell layer; ONL- Outer nuclear layer; OPL. Outer plexiform layer; INL- Inner nuclear layer, IPL- Inner plexiform layer; GCL- Ganglion cell layer.