Desmin (a) and nephrin (b) scores in glomeruli of female αERKO mice, their female wild-type (WT) littermates, ovariectomized female WT (WT-Ovx) and αERKO mice (ERKO-Ovx), and Ovx-B6 mice supplemented with T. Desmin and nephrin staining were measured as described in Materials and Methods and expressed as mean ± s.e.m. (c) Number of TUNEL-positive cells per glomerulus in the different experimental groups (mean ± s.e.m.). Sections were analyzed from 5 mice/group, 10 glomeruli/section. ANOVA with Newman-Keuls’ multicomparison test was performed. (d) Representative images of caspase 3 immunofluorescence staining and colocalization with synaptopodin expression, used as podocyte marker, in female αERKO mice, their female WT littermates, ovariectomized female WT (WT-Ovx) and αERKO mice (ERKO-Ovx), and Ovx-B6 mice supplemented with T. ANOVA, analysis of variance; Ovx, Ovariectomy; T, testosterone.