PI3K promotes the MeJA-induced leaf senescence. Photographs show leaves after incubation with 50 μM MeJA in pi3k mutants (A) or treatment with PI3K inhibitors (B) for 3 d. Photochemical efficiency (C, F), chlorophyll content (D, G), and SAG12 gene expression (E, H) of detached leaves were shown. UBQ10 was used as internal control. Each bar represents three replications. Asterisks (*) indicates significant difference by t test from no MeJA treatment (*, P < 0.05). Hash marks (#) indicate statistically significant differences between indicated samples (P < 0.05; Student’s t test). Fv/Fm = maximum quantum yield of PSII electron transport (maximum variable fluorescence/maximum yield of fluorescence).