Figure 6.
S4B labeling of cellulose in Col-0, cesa3je5, GFP-CESA3, and xxt1 xxt2 guard cells with open or closed stomata. A to D, F to I, K to N, and P to S, Maximum projections of S4B staining pattern in guard cells from rosette leaves. Stomatal opening was induced by 1 µm FC for 2.5 h (A, F, K, and P) or light for 2.5 h (B, G, L, and Q). Stomatal closure was induced by 50 µm ABA for 2.5 h (C, H, M, and R) or dark treatment for 2.5 h (D, I, N, and S). Bar in S = 5 µm. E, J, O, T, and U, Anisotropy quantification of S4B-stained cellulose in guard cells within each genotype (E, J, O, and T) and within each treatment across genotypes (U). Error bars indicate se, and lowercase letters represent significantly different groups (n ≥ 12 guard cell pairs per genotype per treatment; P < 0.05, one-way ANOVA and Tukey’s test). ANOVA was performed within each genotype in E, J, O, and T and within each treatment in U.