Table II. Quantifications of cellulose content in Col-0, cesa3^je5, and GFP-CESA3 plants.

Cellulose content was quantified by the Updegraff method using 23-d-old rosette leaves or by analyzing S4B fluorescence intensity in guard cells from 3- to 4-week-old plants. Values are presented as means ± se, and lowercase letters represent significantly different groups (n ≥ 10 technical replicates from two biological replicates per genotype for the Updegraff method and n ≥ 60 guard cells from three experiments per genotype for quantification of S4B fluorescence intensity; P < 0.05, one-way ANOVA and Tukey’s test). AFU, Arbitrary fluorescence units.

Genotype	Cellulose Content by the Updegraff Method	S4B Fluorescence Intensity in Guard Cells
	µg mg−1	AFU µm−2
Col-0	114.1 ± 4.7 a	574,977 ± 39,350 a
cesa3je5	63.0 ± 3.1 b	318,930 ± 33,171 b
GFP-CESA3	81.4 ± 2.2 c	570,801 ± 43,095 a